Improvement of production rate on recombinant CHO cells in two-stage culture

Background Cultivation temperature is a key environmental parameter that influences cell growth and recombinant protein production. Recombinant CHO (rCHO) cells are usually cultivated at 37 °C. Although lowering culture temperature below 37 °C decrease specific growth rate, in many cases, the specific production rate, q, of CHO cells was not enhanced by lowering the culture temperature. Unlike the specific growth rate, effects of low temperature cultivation on specific productivity rate are not so clear [1]. In the present study, we investigated the effect of low temperature cultivation on rCHO cell growth and production rate. We proposed a two-stage culture that the cultivation was carried out at 37 °C and then a culture temperature become lower. We report that the final production concentration by the two-stage culture is higher than that in case of a flat temperature at 37 °C.